The council will make a submission to the Victorian Commission for Gambling Regulation on February 14 objecting to L'Unico's application to raise the number of electronic gaming machines from 28 to 35 - a 25 per cent increase.

Monash now has 1000 pokie machines, making it the municipality with the highest number in metropolitan Melbourne.

There are 15 pokies venues in Monash, the third highest in the state.

Monash mayor Stefanie Perri said council was certain the extra machines would have a detrimental effect on the community - a key criterion the VCGR uses to decide whether to approve them.

In the 2010-11 financial year, Monash gamblers lost over $122 million on electronic gaming machines. The figure was the third highest in the state.

At the L'Unico Bar and Bistro alone, more than $5million went through the pokies.

The seven extra machines at L'Unico are expected to blow out revenue by another $1.7million or $180,905 per machine.

L'Unico has estimated $763,965 will come from Monash patrons, including Clayton residents, 25 per cent of whom earn less than $500 a week.

Cr Perri said council understood gambling was a lawful activity.

"We understand that there's two sides to the coin," she said. "For many people it's not a problem and that's great, but we also feel a duty of care to those for whom it is a problem. "

Cr Paul Klisaris supported the council's report and said electronic gaming machines had become "a plague on our society".

"Any person who has been inflicted with this gambling disease will you tell you that they can never be cured," he said.

Cr Joy Banerji agreed and said Clayton would suffer. "This is something that we will have to fight," she said.

L'Unico's application also included a promise to donate $200,000 over 10 years to a charity. It also said that regardless of whether the application was successful, a charitable fund would be established.

L'Unico owner Frank Golotta said the bar and bistro was in talks to set up a charity for local children. He said if the application were to be approved, the charity could be more readily set up.

The funds raised by seven extra machines would also fund a revamp of L'Unico to become the Clayton Hotel.